Invitrogen zero blunt manual

Zero Blunt® TOPO® PCR Cloning kits for subcloning provide a highly efficient, 5-minute cloning reaction for the direct insertion of blunt-ended PCR products Shop Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells: Home | Fisher Scientific. Figure 2 presents diagrams of the ligation reactions used in three systems available from Life Technologies for TA cloning, blunt-end cloning and directional blunt-end cloning. Kits are also available for cloning “long” DNAs (1 kb), and for cloning of PCR-derived DNAs in sequencing vectors and in pro and eukaryotic expression vectors. The Zero Blunt PCR Cloning Kit offers an easy method for high efficiency 80 cloning of blunt end PCR products amplified with proof reading thermostable DNA polymerases The Zero Blunt PCR Cloning Kit uses the multipurpose cloning vector pCR Blunt and ExpressLink T4 DNA Ligase to generate a ligation product in a five minute room temperature ligation step Features of the .

Zero Blunt TOPO PCR Cloning Kit options The Zero Blunt TOPO PCR Cloning Kit for direct insertion of blunt-ended PCR products into a plasmid vector can be purchased with a variety of competent cells that deliver different advantages depending upon your needs General cloning: TOP10 cells (Cat. No. K, K) High-efficiency cloning. The final PCR products were cloned with the use of Zero Blunt Topo PCR Cloning Kit (Invitrogen), and the inserts were sequenced using M13 Forward (−20) and M13 Reverse oligonucleotides, following recommendations of the manufacturer High-throughput analysis of the RNA 3′-ends was performed essentially as described previously, with some. For Preparation and Rapid Cloning of Blunt-end DNA for Sequencing Catalog nos. K, K, K, and K A Limited Label License covers this product (see Purchaser Notification). By use of this product, you accept the terms and conditions of the Limited Label License. www.doorway.ru tech_service@www.doorway.ru

The Zero Blunt ® TOPO ® PCR Cloning Kit for Sequencing provides a highly efficient, 5-minute, one-step cloning strategy ("TOPO ® Cloning") for the direct insertion of blunt-end PCR products into a plasmid vector for sequencing. No ligase, post-PCR procedures, or PCR primers containing specific sequences are required. Zero Blunt® TOPO® PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR™-Blunt II-TOPO® Vector for 5-minute benchtop ligations without ligase (1). The Zero Blunt® PCR Cloning Kit offers an easy method for high-efficiency (80%) cloning of blunt-end PCR products amplified with proof-reading, thermostable DNA polymerases. The Zero Blunt® PCR Cloning Kit uses the multipurpose cloning vector pCR™-Blunt and ExpressLink™ T4 DNA Ligase to generate a ligation product in a five-minute, room-temperature ligation step.